Funct. Mater. 2016; 23 (3): 437-442.
Determination of free phenolics and combined phenolics in foliage of Magnolia sieboldii K. Koch
The Provincial Key Laboratory on Forestry and Ecological Environment of Beihua University, Jilin City, 132013, P.R. China
In order to further understand the type and content of phenolics of Magnolia sieboldii K. Koch, the method of high performance liquid chromatography (HPLC) was used for measuring qualitatively and quantitatively free phenolics, combined phenolics and ester phenolics in foliage. Chromatographic conditions: C18 column, Mobile phase A pump 0.5% formic acid aqueous solution, B pump is 0.5% Formic acid methanol solution, The total flow rate of 0.8 ml/min, the column temperature 30C, SPD detector. The experimental results showed that the foliage contained coumaric acid and chlorogenic acid of two kinds of free phenolics, caffeic acid, coumaric acid and catechin coumarin in combined state, coumarin and coumaric acid in ester form, content range was 0.012 ~ 5.31 mg.L-1. Various forms of coumaric acid widely existed in leaves.
1. Ortiz D, Shea T B, J. Alzheimer's Disease, 6,27, 2004.
2. Qi Xiang-yang, Chen Wei-jun, Peng Guang-ua. Acta Nutr. Sinica, 25,397, 2003.
3. Xiangyang Qi, Weijun Chen, Guanghua Peng, Acta Nutr. Sinica, 2003, 25, 4 :397-400.
4. Liping Wang, Shengmao Zhou, Lidan Dai, Acta Agricult. Zhejiangensis, 22 (5), 696, 2010.
5. Xuejiao Feng, Xueli Cao, Yan Li, J. Beijing Techn. Bus. Univ. (Nat. Scie.e Ed. ), 26, 13, 2004.
6. N.W. Albert, D.H. Lewis, H. Zhang, J. Exp. Bot., 60(7), 2191, 2009.
http://dx.doi.org/10.1093/jxb/erp097
7. Yanfang Zhang, Yunhong Yang, Guosui Su, Chem.Analys. Met., 19, 42, 2010.
8. Hongli Nian, He Li, Dongdong Cao, J. Beijing Forestry University , 33, 139, 2011.
9. Hongzhen Sun, Haitao Lu, Suhua Bai, Int. J. Fruit Scie. 29, 929, 2012.
10. Danhong Shen, Xin Lu, Yuwei Chang, Chinese J. Chromatography, 32 (1), 40, 2014.
http://dx.doi.org/10.3724/SP.J.1123.2013.08004
11. Hongli Nian, Weibo Zhang, Ziping Xue, J. Scie. Techn. Food Ind., 30 (11), 65, 2009.
12. Zhonggao Jiao, Jiechao Liu, Hongping Zhou, J. Beijing Techn. Bus. Univ. (Nat. Scie.e Ed. ) 34, 133, 2008
13. Xiaofang Zhao.Studies on the Relationship between Defense substances and Pathogen of Ring Rot Disease onYa Pear.[D] Zheng Zhou, Henan agricultural university, 2008.
14. Xiting Bai, Xuewen Zhu, Lei Luo, J. Scie. Techn.Food Ind., 28, 216, 2007.