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Funct. Mater. 2019; 26 (3): 643-647.

doi:https://doi.org/10.15407/fm26.03.643

Development of assay method for the determination of iodine-containing organic compounds by high-performance liquid chromatography

I.N.Vladymyrova1, V.A.Georgiyants1, V.I.Husarov2, T.S.Tishakova3, D.S.Kharchenko4

1National University of Pharmacy, 4 Pushkinska Str., 61003 Kharkiv, Ukraine
2Pharmtechnology LLC, 22 Korzhenevskogo Str., 220024 Minsk, Belarus
3Kharkiv National Medical University, 4 Nauky Ave., 61022 Kharkiv, Ukraine
4Ukrainian Military Medical Academy, 24 Mel'nykova Str., 04655 Kyiv, Ukraine

Abstract: 

Scientific data on the availability and application of methods for the determination of iodine-containing compounds in herbal raw materials, food products and medicinal products has been analyzed. It has been established that in most methods of iodine detection, an organic component of food interferes analysis. Dry ashing procedure (dry digestion) in muffle furnace at 400-500°C or treatment with concentrated acids (wet digestion) at the presence of oxidizing agents is used to eliminate the potential interference. Inversion voltammetry is used for the iodine determination in food products but it has a number of disadvantages, in particular, toxicity of mercury, its relative easy oxidizability and relative complexity of used equipment. The content of total iodine in herbal raw material is determined by the titrimetric method. The technique has been developed for the determination of tyrosine and its iodinated derivatives by high performance liquid chromatography. The article presents the parameters of chromatographic separation of tyrosine and iodinated derivatives.

Keywords: 
iodine-containing organic compounds, laminaria, high performance liquid chromatography.
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